CUTANA pAG-Tn5 for CUT Tag 試劑

更新時間：2024-05-22

簡要描述：

型號：廠商性質(zhì)：代理商瀏覽量：876

CUTANA pAG-Tn5是靶向剪切及轉(zhuǎn)座酶(CUT&Tag)技術(shù)中進行高效繪制染色質(zhì)特征的關(guān)鍵試劑。與ChIP-seq相比，CUT&Tag在降低細(xì)胞需求量和測序深度的信噪比方面進行了顯著改進。CUTANA pAG-Tn5是一種高活性的E. coli轉(zhuǎn)座酶突變體(Tn5)與蛋白A/G的融合產(chǎn)物，可與來自多種物種宿主的靶抗體兼容。它也沒有表位標(biāo)簽，使其適合于標(biāo)簽介導(dǎo)的CUT&Tag(如FLAG、HA、TY1、V5等)。該產(chǎn)品經(jīng)過高度純化，已去除污染的E. coli DNA，并在細(xì)胞數(shù)量少的情況下進行復(fù)雜分析。為了每次實驗的標(biāo)準(zhǔn)化以及抗體驗證和反應(yīng)監(jiān)測，推薦使用SNAP-CUTANA™ nucleosome spike-ins(如EpiCypher 19-1002)。Tn5帶有mosaic 接頭，可在CUT&Tag中使用。

Adapters

Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’

Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’

Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’

組分

50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

保存條件

Stable for one year at -20℃ from date of receipt. The protein is not subject to freeze/thaw under these conditions.

數(shù)據(jù)示例

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 1:

Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Uncharged pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA, Tn5 dimerizes to a final complex weight of 191 kDa.

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 2:

Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

CUTANA pAG-Tn5 for CUT Tag 試劑

FIGURE 3:

CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control.

訂購詳情

貨號	產(chǎn)品名稱	規(guī)格
15-1017	CUTANA™ pAG-Tn5 for CUT&Tag	50 Reactions
15-1117	CUTANA™ pAG-Tn5 for CUT&Tag	250 Reactions

如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品，請聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物

留言框

產(chǎn)品：
您的單位：
您的姓名：
聯(lián)系電話：
常用郵箱：
省份：
詳細(xì)地址：
補充說明：
驗證碼：

請輸入計算結(jié)果（填寫阿拉伯?dāng)?shù)字），如：三加四=7

上一篇：CUTANA pAG-MNase for ChIC/CUT RUN Workflows

下一篇：Epicypher 13-0041,Histone H3K4me3 Antibody

返回列表>>

欧美日本免费一区二_久久99国产亚洲精品观看_精品少妇一区二区三区在线播放_国产成人高清在线_狠狠操首页_好好操欧美_秋霞黄色毛片_久久精品国内热,亚洲精品国精品久久99热一,亚洲日韩精品无码AV,国产av一区二区精品

CUTANA pAG-Tn5 for CUT Tag 試劑

留言框

產(chǎn)品：

您的單位：

您的姓名：

聯(lián)系電話：

常用郵箱：

省份：

詳細(xì)地址：

補充說明：

驗證碼：