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Adiponectin (human), (recombinant)

更新時(shí)間:2022-01-28

簡(jiǎn)要描述:

產(chǎn)品名稱:Adiponectin (human), (recombinant)
品牌: ENZO產(chǎn)品貨號(hào): ALX-522-063-C050規(guī)格: 1*50µg
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型號(hào):廠商性質(zhì):代理商瀏覽量:1085
  • Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species

  • Expressed in mammalian cell line to guarantee proper post-translational modifications

  • High purity and low endotoxin content to ensure experimental success

Product Details

Alternative Name:Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28

MW:~33kDa (SDS-PAGE).

Source:Produced in HEK 293 cells. Human adiponectin (aa 16-244) is fused at the N-terminus to a linker peptide (14 aa) and a FLAG®-tag.

UniProt ID:Q15848

Concentration:1mg/ml after reconstitution.

Formulation:Lyophilized. Contains PBS.

Purity:≥90% (SDS-PAGE)

Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).

Reconstitution:Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.

Shipping:Shipped on Blue Ice

Long Term Storage:-20°C

Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.

Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Technical Info/Product Notes:Historical results have shown that adiponectin activates AMPK.

FLAG is a registered trademark of Sigma-Aldrich Co.

Regulatory Status:RUO - Research Use Only

<strong>Adiponectin (human), (recombinant)</strong> image
Figure: Superdex 200 analysis of Adiponectin (human) (recombinant) (Prod. No. ALX-522-063) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin. For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References."
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<strong>Adiponectin (human), (recombinant)</strong> image


Product Literature References

Role of adiponectin/peroxisome proliferator-activated receptor alpha signaling in human chorionic gonadotropin-induced estradiol synthesis in human luteinized granulosa cells: T. Tao, et al.; Mol. Cell. Endocrinol. 493, 110450 (2019), Abstract;
Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease: Y. J. Peng, et al.; J. Biomed. Sci. 25, 24 (2018), Abstract; Full Text
Infliximab therapy restores adiponectin expression in perivascular adipose tissue and improves endothelial nitric oxide-mediated vasodilation in mice with type 1 diabetes: C. Nacci, et al.; Vascul. Pharmacol. 87, 83 (2016), Application(s): Immunoblotting, adipose tissues and heart samples; ELISA determination of Ad concentrations, Abstract;
Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells: E.P. 3rd Wickham, et al.; J. Assist. Reprod. Genet. 30, 963 (2013), Abstract; Full Text
Adiponectin and its receptors in the ovary: further evidence for a link between obesity and hyperandrogenism in polycystic ovary syndrome: F.V. Comim, et al.; PLoS One 8, e80416 (2013), Abstract; Full Text
Adiponectin inhibits neutrophil apoptosis via activation of AMP kinase, PKB and ERK 1/2 MAP kinase: A. Rossi, et al.; Apoptosis 18, 1469 (2013), Abstract; Full Text
Adiponectin inhibits neutrophil phagocytosis of Escherichia coli by inhibition of PKB and ERK 1/2 MAPK signalling and Mac-1 activation: A. Rossi, et al.; PLoS One 8, e69108 (2013), Abstract; Full Text
Adiponectin induces CCL20 expression synergistically with IL-6 and TNF-α in THP-1 macrophages: M. Suzuki, et al.; Cytokine 58, 344 (2012), Abstract;
Inhibition of smooth muscle cell proliferation by adiponectin requires proteolytic conversion to its globular form: M. Fuerst, et al.; J. Endocrinol. 215, 107 (2012), Abstract; Full Text
Globular adiponectin counteracts VCAM-1-mediated monocyte adhesion via AdipoR1/NF-κB/COX-2 signaling in human aortic endothelial cells: F. Addabbo, et al.; Am. J. Physiol. Endocrinol. Metab. 301, E1143 (2011), Application(s): WB of human aortic endothelial cells, Abstract; Full Text
Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture: K. Udo, et al.; Kidney Int. 78, 60 (2010), Abstract;
Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation: A.A. Richards, et al.; Mol. Endocrinol. 24, 229 (2010), Abstract;
Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase: A. Suzuki, et al.; Mol. Cell. Biol. 27, 4317 (2007), Abstract;

General Literature References

Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity: U.B. Pajvani, et al.; J. Biol. Chem. 279, 12152 (2004), Abstract; Full Text
ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism: A.H. Berg, et al.; Trends Endocrinol. Metab. 13, 84 (2002), (Review), Abstract;
Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30): T.S. Tsao, et al.; J. Biol. Chem. 277, 29359 (2002), Abstract; Full Text


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