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Epicypher:EpiDyne-FRET Nucleosome Remodeling Assay Substrate

更新時(shí)間:2023-12-14   點(diǎn)擊次數(shù):685次

當(dāng)與酶活性重塑復(fù)合物如SMARCA4、SMARCA2和ACF(如EpiCypher貨號(hào)15-1014、15-1015、15-1013)配對時(shí),該產(chǎn)品提供核小體重塑的高通量熒光讀數(shù)。EpiDyne®-FRET單核小體由在大腸桿菌中表達(dá)的重組人組蛋白(組蛋白H2A-Cy5、H2B、H3.2和H4各兩種;accession numbers::H2A-P4908;H2B-O60814;H3.2-Q71DI3;H4-P62805)組裝而成,該組蛋白由212個(gè)DNA堿基對纏繞,并攜帶5'Cy3、Widom 601核小體定位元件和重塑時(shí)暴露的DpnII限制性內(nèi)切酶基序(GATC)[1]。H2A在殘基120位處具有Thr-Cys取代,其中Cy5是共軛的。H3.2在殘基110位處具有Cys-to-Ala取代。

[1] Lowary & Widom J Mol. Biol. (1998) PMID: 9514715.


產(chǎn)品詳情

保存溫度:Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

運(yùn)輸溫度:Dry ice.

產(chǎn)品形式:Purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg DNA+protein) in 44.2 µL of 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da. Molarity = 4.7 µM.


驗(yàn)證數(shù)據(jù)


Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 1: DNA Gel Data

EpiDyne-FRET substrate resolved via native PAGE gel and 

stained with ethidium bromide to visualize DNA. Lane 1: Free 

DNA (100 ng). Lane 2: Intact EpiDyne-FRET nucleosomes 

(400 ng).

Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 2: Protein Gel Data

Coomassie stained PAGE gel of proteins in EpiDyne-FRET 

substrate (1 µg) demonstrates the purity of histones in the 

preparation. Sizes of molecular weight markers and positions

of the core histones (H2A-Cy5, H2B, H3.2, and H4) are 

indicated.

Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 3: Nucleosome Remodeling Data

ACF1/ATP-dependent nucleosome remodeling reaction. EpiDyne-FRET 

nucleosomes (15 nM) were incubated with ACF1 chromatin remodeler

 (EpiCypher 15-1013; indicated concentrations) in the presence or absence 

of 1 mM ATP. Upon the addition of ATP, reactions were immediately read 

in an Envision Multilabelplate reader (PerkinElmer). Data are presented 

as the mean of the Cy3/Cy5 ratio (N=2).

訂購詳情


貨號(hào)

產(chǎn)品名稱

規(guī)格

16-4201   

EpiDyne-FRET Nucleosome Remodeling Assay Substrate   

50 µg   



Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品,請聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物

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